Comparison of four different infestation techniques for field bioefficacy testing against Asian corn borer, Ostrinia furnacalis (Guenee), in the Philippines
2016
Caasi-Lit, M.T. | Lontoc. M.B.T.
The search for pest-resistant crops, both through classical host plant resistance strategies and by modern genetic modification technology, necessitates bioefficacy testing. Specifically for bioefficacy studies of Bt corn hybrids against the Asian corn borer (ACB), Ostrinia furnacalis (Guenee), infestation is done mainly by manually placing the eggmasses with a pair of forceps or larvae onto the plants with a camel hair brush as well as the bazooka technique. The advantages and disadvantages of these techniques have been discussed by previous authors. Different infestation techniques, namely, the use of camel's hair brush, bazooka, corn stalks and blackhead stage eggmasses were compared to test their effectiveness as delivery systems for neonate ACB larvae for bioefficacy testing. This was done for both greenhouse and field trials. Results showed that corn stalks at 25-30 DAP and blackhead-stage eggmasses at 50 DAP had the highest larval survival. However, using corn stalks and directly infesting the neonates require a lot of labor inputs during preparation. The use of bazooka gave the most inconsistent results due to the quality of the corn grits and the inevitable presence of predators during infestation.
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