Multiple shoot formation and callus induction in Queen pineapple (Anonas comosus (L.) Merr.) shoot tip and leaf explants
2017
Moreno, L.O. | Castillo, D.N. | Duatin, F.M.Y.
Pineapple is commonly propagated using suckers, however, it requires at least 18 months to produce the first crop then suckers growing out from the base of the mother plant could be used as planting materials. With this time gap it would take more time to produce sufficient planting materials to plant an additional area for expansion. Micropropagation technique could allow for efficient and rapid increase of selected varieties. Contamination of cultures was significantly reduced to less than 10% using 10% commercial bleach, 70% alcohol, 10% Chloramphenicol at different soaking time intervals. Multiple shoot formation was induced using 5% BAP, 1% IAA and vitamins after 8 weeks. The average number of shoots formed per culture ranged from 15 to 29 using crown and suckers as explants source, respectively. MS medium supplemented with different concentrations of 2, 4-D induced callus formation on decapitated shoots and leaves of the Queen Pineapple cultures after 4 weeks at the rate of 54 to 100% callus formation, respectively.
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