Investigations on the resistance of commensal swine Escherichia coli to some Aminoglycosides-Aminocyclitols
2015
Urumova, Valentina (Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Infectious and Parasitic Diseases) | Lyutskanov, Mihni (Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Infectious and Parasitic Diseases) | Petrov, Vladimir (Trakia University, Stara Zagora (Bulgaria). Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Infectious and Parasitic Diseases)
Th e aim of this study was to describe the prevalence of antibiotic resi- stance to some aminoglycosides, streptomycin, spectinomycin and genta- micin and three aminoglycoside- resistance genes in Escherichia coli isolated from feces and lagoon manure in six swine farms in Republic of Bulgaria. A total of 274 E. coli isolates from 270 fecal samples and twelve samples from lagoon manure were tested by disk diff usion method to determine resistan- ce patterns to 11 antimicrobial agents. Aminoglycosides resistance also was determined by E-test, agar dilution method, PCR and qPCR. Th e highest resistance observed to streptomycin (70.0%) and spectinomycin (65.5%). Multi-resistance patterns in studied E. coli strains showed that the resi- stance to streptomycin/spectinomycin was most frequently seen together with resistance to ampicillin, tetracycline, and sulfonamides (39.6%). Th e E. coli isolates resistant to streptomycin, spectinomycin were examined for the presence of strA/strB, aadA1 genes, and resistant isolates to gentamicin were evaluated for the presence of the aacC1 gene. Th e most common gene determining resistance to aminoglycosides was aadA1 which was found in 54.0% of swine isolates and lagoon manure isolates followed by straA/strB genes (32.3%). Th e aacC1gene was not identifi ed in E. coli isolates resistant to gentamicin.
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