Uticaj nerasta i godišnje sezone na pojedine parametre fertilizacionog potencijala spermetozoida / Boar and season effects on some parameters of semen fertilizing potential
2016
Apić, Jelena (Naučni institut za veterinarstvo Novi Sad, Novi Sad (Srbija)) | Radović, Ivan (Poljoprivredni fakultet, Novi Sad (Srbija)) | Stančić, Ivan (Poljoprivredni fakultet, Novi Sad (Srbija)) | Vakanjac, Slobodanka (Fakultet veterinarske medicine, Beograd (Srbija))
In order to determine the more accurate fertile potential of sperm, it seems that the conventional parameters of boar semen quality (the ejaculate volume, sperm concentra- tion, progressive motility, percentage of live sperm and of those with intact acrosomal mor- phology) are insufficient. Since recently, there have been numerous studies proving that protein concentration in sperm plasma has high positive correlation with boar fertile po- tential. The research objective was to determine the effect of boars as well as the season on the variation of protein content in the sperm plasma. For the research there were used spermal fractions of 2 boars with high (V-boar) and 2 boars with low (N-boar) protein con- tent in spermal plasma. The ejaculates of boars were taken once a week, for a month, during one year (4 × 12 = 48 ejaculates per boar). For protein analysis in the spermal plas- ma, the samples were prepared by centrifugation. The ejaculate volume, protein concen- tration and progressive motility varied considerably (p < 0.05 or p < 0.01) among the boars as well as in one and the same boar. The variations of the same parameters were deter- mined also during both warm and cold season. On the other hand, protein concentration was rather constant, and in V-boars (ranged from 4 to 4.5%) while in N-boars (ranged from 2.3 to 2.6%). The season did not significantly affect (p > 0.01) protein content in sperm plasma (V-boars: 4 to 4.5% in warm and cold season; N-boars: 2.3 do 2.6% in warm and 2.3 to 2.5% in cold season). The obtained results showed that measurement of protein con- tent in boar sperm plasma could be a useful method for their ranking, based on fertile po- tential of fresh semen.
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