Biological Effects of Glucose Oxidase Gene Transfer in Potato Plants and Stability of Transgenic Plants to Hyperthermia
2016
Grabelnych O. I. | Borovik O. A. | Lyubushkina I. V. | Gamburg K. Z. | Fedyaeva A. V. | Fedoseeva I. V. | Sauchyn D. V. | Urbanovich O. Yu
It is known that the increase of plant tolerance to adverse environmental factors contributes to increasing the concentration of endogenous reactive oxygen species, which are signalling molecules for the launch of protective mechanisms. Introduction and expression in the plant genome heterologous genes, in particular, gox gene, which encodes a glucose oxidase enzyme leads to constant higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we study biological effects of transformation and evaluation of resistance to temperature stress of potato plants with altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the early coming out of dormancy of transformed plants and slower growth rate. Transformants containing the glucose oxidase gene were more sensitive to lethal thermal shock (50 °C 90 min) than the transformant with the empty vector (pBI) or untransformed plants (CK). Pre-heating of plants at 37 ºC significantly weakened the damaging effect of to lethal thermal shock. This attenuation was most significant in the non-transformed plants.
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