PCR Analysis for Halal Authentication of Cosmetics Containing Carrot, Oyster Mushroom, and Pine Needle Extracts
2018
Jang, C.S., Dongguk University, Seoul, Republic of Korea | Kim, T.H., Dongguk University, Seoul, Republic of Korea | Kim, Y.S., Dongguk University, Seoul, Republic of Korea | Lee, B.Z., Chemland Co. Ltd., Anseong, Republic of Korea | Hong, K.W., Dongguk University, Seoul, Republic of Korea
In recent years, interest in halal authentication from the domestic food and cosmetics field has been growing for advances into the overseas halal market. For halal authentication, the product must not contain haram ingredients derived from pig, dog, human, GMO, etc. In this study, the presence of haram ingredients in plant extracts (carrot, oyster mushroom, and pine needle) treated with papain and bromelain and cosmetics (mask pack and cream) containing these extracts were analyzed by PCR to confirm whether these cosmetics were suitable for halal authentication. Detection limits of the PCR method that specifically detected template DNA of human, pig, dog, and GMO were 1.29×10³ , 1.14×10³ , 1.24×10² and 2.02×10³ copies/tube, respectively. PCR was not inhibited by the plant extracts or cosmetic ingredients. Results of PCR for the plant extracts or cosmetics containing these extracts were all negative. This PCR method could be used to rapidly identify the presence of haram ingredients in raw materials or final products during the manufacturing process of food and cosmetics.
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