Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea
2018
Kim, J.Y., Seoul Women's University, Seoul, Republic of Korea | Jang, J.H., Kyungpook National University, Daegu, Republic of Korea | Park, J.H., Catholic Kwandong University, Incheon, Republic of Korea | Jung, H.Y., Kyungpook National University, Daegu, Republic of Korea | Park, J.S., Chungbuk National University, Cheongju, Republic of Korea | Cho, S.J., Catholic Kwandong University, Incheon, Republic of Korea | Lee, H.B., Seoul Women's University, Seoul, Republic of Korea | Savitree Limtong, Kasetsart University, Bangkok, Thailand | Gayathri Subramani, Seoul Women's University, Seoul, Republic of Korea | Sung, G.H., Catholic Kwandong University, Incheon, Republic of Korea | Kim, M.K., Seoul Women's University, Seoul, Republic of Korea
Grasshoppers play vital role in the digestion of photosynthetically fixed carbons. With the aid of intestinal microflora, the grasshopper can degrade leaves constituents such as cellulose and hemicellulose. The purpose of this study was to examine cellulolytic yeast isolates from the gut of grasshoppers collected in Gyeonggi Province, South Korea. Among the yeast isolates, ON2, ON17 (two strains), and ON6 (one strain) showed positive cellulolytic activity in the CMC- plate assay. The sequence analyses of D1/D2 domains of the large subunitrDNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON2 and ON17 were most closely related to Papiliotrema aspenensis CBS 13867T (100%, sequence similarity in D1/D2 domains; 99.4% sequence similarity in ITS) and strain ON6 related to Saitozyma flava (100% in D1/D2 domains; 99.0% in ITS). All these three yeast strains are capable of degrading cellulose; therefore, the members of endosymbiotic yeasts may produce their own enzymes for carbohydrate degradation and convert mobilized sugar monomers to volatile fatty acids. Thus, the endosymbiotic yeast strains ON2, ON17 (represents the genus Papilioterma) and ON6 (Saitozyma) belonging to the family Tremellomycetes, are unreported strains in Korea.
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