Determination of Nitrovin in Fishery Products by Liquid Chromatography-tandem Mass Spectrometry
2018
Kim, J., National Institute of Food and Drug Safety Evaluation, Cheongju, Republic of Korea | Shin, D., National Institute of Food and Drug Safety Evaluation, Cheongju, Republic of Korea | Kang, H.S., National Institute of Food and Drug Safety Evaluation, Cheongju, Republic of Korea | Jeong, J., National Institute of Food and Drug Safety Evaluation, Cheongju, Republic of Korea | Rhee, G.S., National Institute of Food and Drug Safety Evaluation, Cheongju, Republic of Korea
The objective of this study was to develop a sensitive method for the identification and determination of nitrovin in fishery products by using a solid-phase extraction (SPE), as performed with a liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with a mixture of acetonitrile and water, and were then defatted with acetonitrile saturated hexane, after which further clean-up was accomplished with SPE on the hydrophilic-lipophilic balance (HLB) cartridges. The analytes were subsequently ionized in the positive mode of an electrospray ionization (ESI), and where thereby detected in a process of multiple reaction monitoring (MRM). The linearity (expressed as correlation coefficients) of the matrix calibration curves was less than 0.985. The limit of the quantification for the nitrovin was measured at 0.001 mg/kg. The accuracy (expressed as average recovery) was noted between 72.1 and 122%. The precision (expressed as coefficient variation) was noted from 2.9 to 16.9%. According to the CODEX CAC/GL-71 guideline accuracy, precision, linearity, and limit of detection were determined in three matrices (which were flatfish, eel and shrimp). The proposed method was suitable for analyzing the associated nitrovin residues. This application and result can also be a factor to contribute to the non-detection drugs management in fishery products.
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