Development of an Indirect Enzyme-Linked Immunosorbent assay for Rapid Detection of Adulteration of Food Allergen Mackerel in Processed Marine Foods
2018
Lee, J.E., Gyeongsang National University, Jinju, Republic of Korea | Kim, A.Y., Gyeongsang National University, Jinju, Republic of Korea | Kim, S.A., Gyeongsang National University, Jinju, Republic of Korea | Kim, H.I., Gyeongsang National University, Jinju, Republic of Korea | Park, J.H., Gyeongsang National University, Jinju, Republic of Korea | Shim, W.B., Gyeongsang National University, Jinju, Republic of Korea
The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.
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