Antioxidant capacity and hepatoprotective effect of ethyl acetate fraction from shoot of Aralia elata on alcohol-induced cytotoxicity
2018
Kwon, B.S., Gyeongsang National University, Jinju, Republic of Korea | Park, S.K., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.M., Gyeongsang National University, Jinju, Republic of Korea | Kang, J.Y., Gyeongsang National University, Jinju, Republic of Korea | Park, S.H., Gyeongsang National University, Jinju, Republic of Korea | Kang, J.E., Gyeongsang National University, Jinju, Republic of Korea | Lee, C.J., Gyeongsang National University, Jinju, Republic of Korea | Park, S.B., Gyeongsang National University, Jinju, Republic of Korea | Yoo, S.K., Gyeongsang National University, Jinju, Republic of Korea | Lee, U., National Institute of Forest Science, Seoul, Republic of Korea | Heo, H.J., Gyeongsang National University, Jinju, Republic of Korea
To evaluate physiological effect of Aralia elata, in vitro antioxidant activity and hepatic protective effects were investigated. Ethyl acetate fraction from Aralia elata (EFAE) had higher total phenolic content than other fractions (nhexane, chloroform, and distilled water layers). EFAE also showed significantly greater radical scavenging activity against 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), than other fractions. Moreover, EFAE showed dose-dependent inhibitory effect of malondialdehyde (MDA). Hepatoprotective effects of EFAE against ethanol- and H2O2-induced oxidative stress and cytotoxicity in H4IIE and HepG2 hepatic cells were examined using 2',7'-dichlorofluorescein diacetate (DCF-DA) and 3-[4,5-dimethythiazol-2-yl]-2,5- diphenyl tetrazolium bromide (MTT) assay. The results showed that EFAE reduced cellular oxidative stress, and increased hepatic cell viability. In addition, EFAE inhibited ethanol-induced lipid accumulation in HepG2 cells. Finally, physiological substances of EFAE were analyzed using high performance liquid chromatography (HPLC), and the major bioactive compounds identified were 3,5-dicaffeoylquinic acid and chlorogenic acid.
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