Callus induction and plant regeneration from leaves of peony
2018
Xiangtao Zhu, Zhejiang A and F University, Zhuji Zhejiang, People's Republic of China | Xueqin Li, Zhejiang A and F University, Zhuji Zhejiang, People's Republic of China | Wenjie Ding, Zhejiang A and F University, Zhuji Zhejiang, People's Republic of China | Songheng Jin, Zhejiang A and F University, Zhuji Zhejiang, People's Republic of China | Yan Wang, Chinese Academy of Forestry, Beijing, People's Republic of China
Tree peony (Paeonia sufruticosa Andr.) is a valued ornamental plant. This study reports on peony callus induction, shoot organogenesis and plant regeneration using young peony leaves as explants. Various media containing diverse plant growth regulators were assessed for their potency in peony propagation. After exposure of dark-adapted leaf discs to 30 μmol m−² s−¹ of light, inoculation in Murashige and Skoog (MS)+0.2 mg L−¹ 2,4-dichlorophenoxyacetic acid (2,4-D)+0.2 mg L−1 a-naphthaleneacetic acid (NAA)+3.0 mg L−¹ thidiazuron (TDZ) medium resulted to the highest callus induction rate with values reaching up to 87.8%. We identifed that MS+0.2 mg L−¹ NAA+2.0 mg L−¹ 6-benzyladenine (6-BA)+2.0 mg L−¹ kinetin (KT), with a multiplication coefcient of 3.025, to be the optimal medium for further callus proliferation under light. Inoculation in MS+2.0 mg L−16-BA+0.2 mg L−¹ NAA+0.3 mg L−¹ TDZ medium allowed 22.22% of callus cultures to diferentiate into adventitious shoots, whereas a similar rate of root formation was detected when 1/2 MS +0.1 mg L−¹ NAA +0.05 mg L−¹ IBA +30 g L−¹ sucrose medium was used. Our fndings provide important information on peony regeneration and present a new method for peony tissue culture that will potentially facilitate mass propagation and genetic engineering of peony plants.
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