Enhanced Production of beta-D-glycosidase and α-L-arabinofuranosidase in Recombinant Escherichia coli in Fed-batch Culture for the Biotransformation of Ginseng Leaf Extract to Ginsenoside Compound K
2018
Kim, T.H., Konkuk University, Seoul, Republic of Korea | Yang, E.J., Konkuk University, Seoul, Republic of Korea | Shin, K.C., Konkuk University, Seoul, Republic of Korea | Hwang, K.H., Skin Research Institute, Amorepacific Corporation R and D Center, Yongin, Republic of Korea | Park, J.S., Skin Research Institute, Amorepacific Corporation R and D Center, Yongin, Republic of Korea | Oh, D.K., Konkuk University, Seoul, Republic of Korea
Ginsenoside compound K is an essential ingredient in nutritional supplements, cosmetics, and traditional medicines. However, cultivation for the production of enzymes involved in ginsenoside biotransformation has not been attempted in a fermenter. The host strain Escherichia coli ER2566 and the constitutive pHCE vector were selected for the efficient production of β-D-glycosidase, and expression medium composition to produce Sulfolobus solfataricus beta-glycosidase expressed in E. coli was optimized in flask and batch cultures. The total activity of beta-Dglycosidase in fed-batch culture using a fermenter increased 14-fold before optimization. S. solfataricus beta-D-glycosidase and Thermotoga petrophila alpha-L-arabinofuranosidase were produced in a fed-batch culture. These two enzymes completely converted protopanaxadiol-type ginsenosides in ginseng leaf extract obtained from discarded ginseng leaves as a renewable substrate to compound K. The effective bioprocess for compound K production developed here will contribute to the industrial biological production of compound K.
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