OBTAINING PEA INTERSPECIFIC HYBRIDS FOR USE IN MARKER ASSISTANT SELECTION ON HIGH PROTEIN QUALITY
2019
Bobkov, S.V. | Selikhova, T.N.
Unique bands of convicilin identified in P. fulvum accessions k-2523 and k-6070 (N.I. Vavilov All-Russian Institute of Plant Genetic Resources) would be used as biochemical markers for fast producing of breeding lines with high quality of isolated protein suitable for obtaining innovative food products. Pea variety Stabil, introgressive lines ВС2F5 Stabil × i-609881 and l-83-1, breeding lines PAP 485/4 and LU 153-06 were used as cultural components in interspecific crosses. Interspecific hybridization was conducted in 2017 and 2018; the total number of crosses was 49. Pea interspecific hybrids F1 were obtained in crosses PAP 485/4 × k-6070, Stabil × k-2523 and k-2523 × l-83-1. Hybrid plant F1 PAP 485/4 × k-6070 was grown in greenhouse and 13 hybrid seeds F2 were obtained. After dehulling hybrid seeds F2 PAP 485/4 × k- 6070 were splitted into two cotyledons for marker breeding with using unique electrophoretic bands of convicilin. One cotyledon was used for SDS-PAGE electrophoresis. The second cotyledon with intact bud after sprouting was placed in polypropylene vessel with soil for plant growth. Selection of hybrid plants with introgressive isoforms of convicilin was conducted according the data obtained from SDS-PAGE electrophoresis of the first cotyledon.
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