Zn and Cr (VI) Uptake by Bacteria Arthrobacter globiformis 151B from the Potassium-Rich Nutrient Medium
2019
Arthrobacter globiformis 151B is a chromium-resistant bacterium having the ability to maintain viability in the habitat of a large number of Cr(VI) ions. Arthrobacter globiformis 151B bacteria can absorb hexavalent chromium [Cr(VI)] ions from the environment, convert them into trivalent form [Cr(III)] and accumulate them. Because of such properties of Arthrobacter globiformis 151B bacteria, they can be used for detoxification of the environment polluted by highly toxic Cr(VI). Arthrobacter globiformis 151B bacteria can absorb the ions of different metals from the nutrient medium. Macroelements (K, Na, Ca, Si) of the nutrient medium can affect the absorption of metal ions. The process of Cr(VI) and Zn assimilation by chromium-resistant bacteria (Arthrobacter globiformis 151B) and the influence of high concentration of K ions on the mentioned process are studied in the paper. The strain of the bacteria under investigation was isolated from basalt samples taken from the sites highly contaminated with Cr (VI) in Kazreti. Introducing the solutions of Cr, Zn and K into the nutrient medium, we studied the influence of different concentrations of K-ions on the process of Cr and Zn assimilation by bacteria in different cultivation periods of bacteria (17 h, 24 h, 48h, 96h, 144h). The concentration of K added to nutrient medium was 0.6 mg/ml, 2.0 mg/ml and 5.0 mg/ml. After cultivation of the bacteria the precipitation of cells was carried out by centrifuging and the obtained bacterial pellet was prepared for determining the content of metals (Cr and Zn) in the cell by the use of atom-absorption spectrometry. © 2019 Bull. Georg. Natl. Acad. Sci.
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