Expression study of an Amino Acid Permease-like gene in Phaseolus vulgaris L.
2020
Brototi Chakraborty (Laboratory of Genomics of Plant Stress Biology, Department of Biotechnology, Visva Bharati, Santiniketan 731 235, India) | Nisha Patwa (USDA-ARS, Horticultural Insects Research Lab, Application Technology Research Unit, Wooster, OH 44691, USA) | Jolly Basak (Laboratory of Genomics of Plant Stress Biology, Department of Biotechnology, Visva Bharati, Santiniketan 731 235, India)
Amino acid permease-like (AAP-like) gene plays a critical role in absorbing amino acids through roots in plants. A number of studies have been done on amino acids uptake in plants but till date there is no report about the expression of AAP gene in Phaseolus under field allied condition. The aim of this study is to measure the expression of AAP-like gene on alanine, glycine and proline amino acid uptake capacity in Phaseolus vulgaris at field relevant concentrations. Amongst three amino acids, a drastic significant increase of 63.15 fold in expression of AAP-like gene is observed in 50 µM alanine at 2 hr. At 50 µM of proline and 25 µM of alanine, AAP-like gene expression also shows high expression of 43.71 fold at 2 hr and 42.50 fold at 1 hr respectively. This study elucidated the dose dependent relationship of glycine, alanine and proline with the expression of AAP-like gene in amino acid transport in natural conditions in roots of P. vulgaris. Additionally, this research is also useful in identification of plants needing less surplus nitrogen additions and helpful in optimizing fertilizers by tailoring AAP gene expression to match plant uptake capacities in agriculture.
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