Susceptibility genes : an additional source for improved resistance
2017
Sun, Kaile
Potato is affected by several diseases. Although, resistance can be obtained by introgression of major resistance genes from wild species, this has rarely been durable. Hence, other sources of resistance are highly needed. New research with a focus on loss of function mutations has led to the identification of disease susceptibility (<em>S</em>) genes in plants. The research in this thesis was aimed at the identification and characterization of potato <em>S</em> genes involved in the interaction with <em>Phytophthora infestans </em>and <em>Botrytis cinerea</em>. We selected 11 <em>Arabidopsis thaliana</em> <em>S</em> genes and silenced their potato orthologs by RNAi in the potato cultivar Desiree. The silencing of six genes resulted in resistance to <em>P. infestans</em>. Moreover, silencing of <em>St</em><em>DND1 </em>reduced the infection of <em>B.</em> <em>cinerea</em>. Microscopic analysis showed that spore attachment and/or germination of <em>P. infestans</em> and <em>B.</em> <em>cinerea</em> was hampered on the leaf surface of <em>St</em><em>DND1</em>-silenced potato plants. On <em>StDMR1</em>- and <em>StDMR6</em>-silenced potato plants, hyphal growth of <em>P. infestans</em> was arrested by the hypersensitive response-like cell death. Our results demonstrate that impairment of plant <em>S</em> genes may open a new way for breeding potatoes with resistance to pathogens like <em>P. infestans</em> and <em>B. cinerea</em>.
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