Screening for anti-fungal proteins in pearl millet inbreds active against Pyricularia grisea
2019
s marri | m govindaraj | r sharma
Pearl millet is a commonly grown nutri-cereals in the semi-arid regions of India and Africa.Productivity of this crop is challenged by many biotic stress and blast (Pyricularia grisea)has emerged as a new threat. Recurrent chemical protection is expensive and not sustainableapproach for farmers. Therefore, identification and utilization of host plant resistance is veryappropriate. Present study screened 20 inbreds crude proteins for anti-fungal activity againstblast isolates collected from Patancheru (Pg45), Jaipur (Pg138) and Aurangabad (Pg186)using oatmeal agar plates (qualitative) and microspectrophotometric assays (quantitative).There was significant reduction in growth rate of fungal colony of highly virulent isolate Pg138 (9-57%; P<0.01)on oatmeal agar plates at fixed time intervals against Pg138, while nosignificant differences were observed for Pg 45 and Pg 186.Further, inhibition of fungalspore germination and initial growth (48h) of these isolates was monitored quantitatively(595nm) in the presence of each crude protein extract for 48h. As a result, high reduction infungal growth was observed for Pg138 (64-87%) followed by Pg186 (21-61%) and Pg45 (2-31%). Total protein in the tested lines varied 10-15%. Results showed that these proteinextracts were effective (9-50%) against cysteine protease, papain commercially sourcedfrom papaya that indicates millet protein extracts contain cysteine protease inhibitors andnot a trypsin inhibitor. The result suggest that these PI may be used as potential alternativeto anti-fungal agents, and merits further studies contributing to resistance breeding
Show more [+] Less [-]S Marri, R Sharma, M Govindaraj, 'Screening for anti-fungal proteins in pearl millet inbreds active against Pyricularia grisea', pp.222-223, 2019
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by GARDIAN