Inhibition of salmonella binding to porcine intestinal cells by a wood-derived prebiotic
2019
Božić, A. (Faculty of Agriculture, Novi Sad (Serbia)) | Anderson, R. (United States Department of Agriculture, Texas (USA). Agricultural Research Service, Southern Plains Agricultural Research Centre) | Crippen, T. | Hume, M. | Beier, R. | He, H. | Genovese, K. | Poole, T. | Nisbet, D. (United States Department of Agriculture (USA). Agricultural Research Service)
Salmonella can cause disease and contamination of animal-produced foods. Concern that antimicrobial resistant bacteria in animal agriculture may render antibiotics ineffective motivates the development of non-antibiotic strategies to control animal diseases. Oligosaccharide-rich products capable of blocking pathogen adherence to the animal’s intestinal mucosa are attractive alternatives to antibiotics as these can prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucosegalactose- mannose-xylose oligomers was tested for its ability to prevent binding of a green fluorescent protein-labeled Salmonella Typhimurium (GFP-ST) to intestinal porcine epithelial cells (IPEC) cultured in vitro. We found that GFP-ST suspensions (106 and 107 cells/mL) pre-exposed (10 min) to 16 mg of the prebiotic bound 32 to 34% fewer (P < 0.05) individual IPEC cells within monolayers examined by phase contrast microscopy than did GFP-ST suspensions having no prebiotic exposure (the latter binding on average 36.8 and 78.3% IPEC within 4 replicate fields, respectively). Quantitatively, we counted 90% fewer (P < 0.05) prebiotic-exposed GFP-ST cells bound per individual IPEC cell compared to counts obtained for non-prebiotic exposed GFP-ST, the latter averaging 0.26 to 13.77 GFP-ST cells/IPEC cell, respectively. These results suggest anti-adhesion compounds present within the prebiotic inhibited Salmonella’s ability to bind IPEC.
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