Content of malondialdehyde and activity of enzyme glutathione-S-transferase in the leaves of emmer wheat under the action of herbicide and plant growth regulator

The article presents the results of vegetation experiment on the influence of differentrates of herbicide Prima Forte 195 – 2-ethylhexyl ether 2.4-D + aminopyralid + florasulam (0.5;0.6 and 0.7 L ha-1) under different application methods of plant growth regulator of a naturalorigin Wuxal BIO Vita (Ascophyllum nodosum extract + microelements) on the content ofmalondialdehyde (MDA) as an important indicator of the intensity of peroxide oxidation of lipidsin plants and on the activity of enzyme glutathione-S-transferase (GST) in the leaves of emmerwheat.Experimental scheme included 16 experimental variants: 1 – without application of preparationsand pre-sowing treatment of seeds (control); 2, 3, 4 – Prima Forte 195, applied to vegetativeplants at the rates 0.5, 0.6 and 0.7 L ha-1; 5 – Wuxal BIO Vita at the rate of 1.0 L ha-1, applied tovegetative plants; 6, 7, 8 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1in tank mixtureswith Wuxal BIO Vita at the rate of 1.0 L ha-1, sprayed on vegetative plants; 9 – pre-sowingtreatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1(background); 10, 11, 12 – Primaforte 195, applied to vegetative plants at the rates of 0.5, 0.6 and 0.7 L ha-1at the background ofpre-sowing treatment of seeds with Wuxal BIO Vita at the rate of 1.0 L t-1; 13 – Wuxal BIO Vitaat the rate of 1.0 L ha-1(applied to vegetative plants, treated before sowing with Wuxal BIO Vitaat the rate of 1.0 L t-1); 14, 15, 16 – Prima Forte 195 at the rates of 0.5, 0.6 and 0.7 L ha-1respectively, in tank mixture with Wuxal BІО Vita at the rate of 1.0 L ha -1 –spraying of plantsand pre-sowing treatment of seeds with Wuxal BІО Vita 1.0 L t-1.It has been found that under the application of Prima Forte 195 the redox state in the leaves ofemmer wheat increased considerably in the direction of increasing the content of MDA. It hasalso been proved that herbicide Prima Forte 195 and its mixtures with plant growth regulator(PGR) Wuxal BIO Vita caused the changes in the activity of enzyme glutathione-S-transferase,which slightly increased in the initial period in the variants of integrated application of herbicideand plant growth regulator. However, later the GST activity in the variants of integratedapplication of herbicide and PGR decreased, which indicates stabilization and a positive influenceof integrated application of preparations on the detoxification processes in the crops of emmerwheat.