Multiscale characterization of the in vitro digestion of a milk-based emulsion
2014
Bourlieu-Lacanal, Claire | Claude, Mathilde | Ménard, Olivia | Deglaire, Amélie | Bouhallab, Said | Dupont, Didier | Michalski, Marie-Caroline
The luminal phase of lipid digestion consists in a succession of physical (emulsification, aggregation, solubilization, micellarization…) and biochemical modifications (enzymatic hydrolysis of molecules). These modifications take place in various digestive compartments and under the action of a large set of lipolytic enzymes having welldefined substrate selectivity (gastric lipase, pancreatic triglyceride lipase, bile salt stimulated lipase, phospholipase A2 …). This complex phenomena is approached using dynamic or static models of digestion.[br/] Static models (with constant ratio food/simulated digestive fluids for a given compartment) are considered as effective tools to screen a high number of experimental conditions though they reproduce less precisely the transient conditions occurring in vivo. Static models present the main advantage of using a small amount of substrate, enzymes and simulated fluids.
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