Mitochondrial genotyping of isolates of the arbuscular mycorrhizal fungus "<em>Glomus intraradices</em>" from the isolate collection of the International Bank of Glomeromycota
2011
Stockinger, Herbert | Peyret Guzzon, Marine | Thiery, Odile | Gianinazzi-Pearson, Vivienne, | Redecker, Dirk
The culture collection of the International Bank of Glomeromycota (BEG) provides an important service to scientific community and industry by acting as a repository for well-defined AMF isolates which are made available to the public. The most widely-used species has been referred to as "Glomus intraradices", and was recently renamed Rhizophagus irregularis. This species has emerged as a model organism for experimental research and is the subject of the first AMF genome to be sequenced. The only genetic marker that can currently be used to distinguish strains of R. irregularis in field settings is the mitochondrial large subunit (mtLSU). In addition, genetically distinct strains of this species have been demonstrated to possess different symbiotic properties. In order to trace and recognize the different BEG isolates of this species, we characterized them genetically using the high-resolution molecular marker mtLSU. The results showed that the seven isolates we characterized from the collection corresponded to seven different mtLSU haplotypes.
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