Impact of insulin treatment during post-insemination mid-luteal phase on luteal profile and conception rate in buffaloes
2010
L, LALTHAZUALI | SINGH, JAGIR | GHUMAN, S P S | PANDEY, A K | DHALIWAL, G S
The aim of present trial in buffaloes was to test the impact of subcutaneous administration of insulin (long acting purified bovine insulin @ 0.2 IU/kg body weight/day) during post-insemination mid-luteal phase (days 8–12) on luteal profile and conception rate. A set of 22 estrus synchronized (2 PGF2α injections 11 days apart) post-partum buffaloes were assigned to insulin (n=12) or control (n=10) group. All the buffaloes were artificially inseminated (AI) 8–12 h after the onset of estrus and thereafter AI was repeated once at 12 h interval. Transrectal ultrasonography and jugular vein blood sampling was carried out on day 0 (day of last AI), 5, 8, 12, 15, 21 and 40 post-AI. First service conception rate was assessed by transrectal ultrasonography on day 40 post-AI. Data of insulin group showed no alteration in corpus luteum size (P>0.05), although post-treatment plasma progesterone and first service conception rate was higher (P<0.25) in insulin group compared to control group. In summary, insulin administration during post-AI mid-luteal phase may have a beneficial impact on conception rate in buffaloes.
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