Use of flow cytometry for producing tetraploids in red clover
2020
Rebane, A., Latvia Univ. of Life Sciences and Technologies, Skriveri, Skriveri Municipality (Latvia). Inst. of Agronomy | Grauda, D., University of Latvia, Salaspils (Latvia). Inst. of Biology | Rancane, S., Latvia Univ. of Life Sciences and Technologies, Skriveri, Skriveri Municipality (Latvia). Inst. of Agronomy | Jansons, A., Latvia Univ. of Life Sciences and Technologies, Skriveri, Skriveri Municipality (Latvia). Inst. of Agronomy | Rashal, I., University of Latvia, Salaspils (Latvia). Inst. of Biology
Sprouts of diploid red clover ‘Stendes Agrais’ were treated with 0.2% colchicine solution. The ploidy level of plants was detected by flow cytometry. Most of the treated plants were mixoploids and sterile. From progenies of fertile plants, mixoploids were selected that had a proportion of tetraploid cells higher than 60%. In the next generation, stable tetraploid plants were selected, and 49 lines were included in the breeding process.
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