Single locus microsatellite development for the Malaysian giant freshwater prawns, Macrobrachium rosenbergii
2005
Zainoddin Jamari | Rosly Hassan | Siti Shapor Siraj | Subha Bhassu | Khatijah Yusoff | Tan, S. G.
Prawns of the genus Macrobrachium Bate, 1868 (Crustacea: Palaemonidae) are a highly diverse group of decopodcrustaceans found in circumtropical marine-, estuarine- and freshwaters. At present, wild stocks have been used tocultivate commercial cultures in many areas in Malaysia. Such repeated harvesting from wild stocks, however, willeventually lead to extinction and loss of genetic diversity. Therefore, it is important to know the genetic make up of thesewild stocks in order to manage the populations for conservation and breeding purposes. In the present study, a novelmethod known as Random Amplified Microsatellites (RAMS) was used to develop single locus microsatellite markers. Atotal of 13 microsatellite regions were detected. The findings of this study showed a successful detection of the microsatellitesequences in M. rosenbergii using RAMS. This study will be continued to identify more clones that contain microsatellitesequences and to design the forward and reverse primers for each microsatelite region. M. rosenbergii sequences werehomologous with Danio rerio (Zebra fish) and Perna viridis (green lipped mussel).
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