Optimization of cryopreservation for sterculia cordata zygotic embryos using vitrification techniques
2007
M. Marzalina | H. J. Staines | B. Krishnapillay | J. Nadarajan | E. E. Benson | K. Harding
Vitrification is a physical process by which a concentrated aqueous solution solidifies into stable amorphous glass at a sufficiently low temperature. This process eliminates the potentially damaging effect of intra- and extracellular crystallization in cryopreservation of plants and animal cells. Sterculia cordata embryos were cryopreserved using a vitrification technique. The embryos were precultured for three days on MS media supplemented with sucrose (0.35, 0.55 and 0.75 M) and treated with loading solution (LS) and plant vitification solution 2 (PVS2) for one to three hours before storing in liquid nitrogen. Their germinationwas assessed before and after cryopreservation. Multiple logistic regression analysis showed that the sucrose pregrowth medium, LS and PVS2 pretreatments were not detrimental to the germination of embryos. However, sucrose and LS pretreatments alone were not sufficient for successful cryopreservation in contrastto the high germination (≥ 80%) achieved with PVS2 treatment.
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