Tocotrienols reduce monocytes adhesion to stimulated human endothelial cells
2017
Abdul Manaf Ali | Froemming, Gabriele R .A. | Hapizah Md Nawawi | Suhaila Abd Muid | Thuhairah Hasrah Abdul Rahman
The anti-atherosclerotics activity of tocotrienols (TCT) compared to alpha-Tocopherol (α-TOC) in vitro study is not much being reported especially in human endothelial cells. The aim of the present study was to study the effects of TTMF, TCT and α-TOC on monocytes adherence to stimulated endothelial cells and to investigate the correlation between monocytes adherence and adhesion molecules in endothelial cells treated with TTMF, pure TCT isomers and α-TOC. Human umbilical vein endothelial cells (HUVECs) were incubated with TTMF, TCT isomers and α-TOC (0.3-10 µM) together with lipopolysaccharide, LPS (1 μg/ml). Monocytes adherence was measured by Rose Bengal staining. Soluble ICAM-1, VCAM-1 and e-selectin and NFκB binding were measured by ELISA. TTMF and TCT isomers inhibit monocytes adhesion to LPS-stimulated HUVECs but not α-TOC. δ-TCT exhibit the highest % inhibition of monocytes adhesion compared to the other TCT isomers. Only TCT isomers show positive correlation of monocytes adhesion with certain adhesion molecules and NFκB but not TTMF and α-TOC. In conclusion, TTMF and TCT isomers exhibit reduction of adhesion of monocytes to LPS stimulated endothelial cells. The reduction of monocytes adhesion by TCT isomers especially δ-TCT are positively correlated with reduction of adhesion molecules and NFκB deactivation. It can be suggested that TCT especially the δ-TCT isomers is beneficial in the prevention of early atherogenesis in human.
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