Evaluation of cellulase activity from Trichoderma spp. and Xylanolytic bacteria
2014
Salma, S. | Sarmiad, W. | Susilowati, D. N. | Lestari, P. | Richana, N | Suryadi, Y.
Fungi and bacteria were considered as potential decomposers on various substrates. This study was aimed to evaluate the ability of Trichoderma spp. to degrade various cellulolytic substrates and xylanolytic bacteria to decompose lignocelluloses from rice straw. Six selected Trichoderma spp. isolates showed high activities of specific celullase in given substrates. Pan23.2 isolate was shown to be superior in all cellulose substrates without glucose with the highest activity to hydrolyze cellulose crystals. Some isolates preferentially hydrolyzed crystalline (Pan23.2, Bo17, Andi2.1) while others (Kun4, Gam4.1, Pan23.1) were more dominant on amorph hydrolysis. Not all cellulases from Trichoderma spp. with high activity on cellulose amorph showed activity on cellulose crystals. The addition of glucose into cellulose substrates increased the cellulase productivity of Andi2.1 and Gam4.1 isolates. Combination of 1% glucose and 1% cellobiose were the best substrate for CMCase activity, while 1% glucose and 1% CMC were good for avicelase. However, xylanolytic bacteria had no ability to degrade lignocelluloses on rice straw which was indicated by low temperature and high C/N ratio during composting process. In this study, all Trichoderma spp. isolates demonstrated high potency as decomposers, but the xylanolytic bacteria may be used for other applications other than as decomposers.
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