Cell viability assay guided fractionation of natural rubber latex sera
2013
Yang, K. L. | Norazreen Abd Rahman | Ong, M. T. | Sunderasan E. | Lam, K. L.
Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionation of latex B- and C-serum using cell viability assay (anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B- and C-serum against distilled water caused some of its constituents to precipitate, then a brief centrifugation separated their fractions – dialysed B-serum precipitate (DBP), dialysed B-serum supernatant (DBS), dialysed C-serum precipitate (DCP) and dialysed C-serum supernatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B- and C-serum precipitate from their supernatant. Cell viability assay performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B- and C-serum fractions. Chromatographic separation of DBP, DCP and DCS followed by cell viability assay will shed light if sub-fractions with further improved anti-proliferative activity are attainable.
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