Measurements of total extractable proteins in latex gloves: a comparative study of the RRIM and ASTM tests
1997
Esah Yip
Extractable protein contents of latex gloves generated by two commonly used methods, the RRIM (MS 1392:96P) and the ASTM (D 5712-95) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRJM, determined by the RRIM test, ranged from 1326 m/g to 20 mg/g for 90 gloves. Their corresponding EPASTM values, obtained by the ASTM test, varied from 1377 mg/g to < 50 mg/g. Statistical analysis showed a very significant correlation between them+ , with a coefficient of correlation, r = 0.93, P < 0.001. Generally, EPRRJM values read higher than those of EPASTM. EPASTM of 50 mg/g and lower were found to be associated with EPRRJM values ranging from 267 mg/g to < 20 mg/g, suggesting higher sensitivity of the latter measurements. Relevance of the two sets of EP in relation to the allergenicity/allergic potential of latex gloves was discussed. Accelerated ageing at 70°Cfor 7 days of latex gloves resulted in the lowering of protein contents. The effect appeared to be more pronounced for EPRRJM, than for EPASTM.
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