Monoclonal antibodies identify different antigens in proteins eluted from natural rubber latex gloves obtained from different sources
1994
Cardosa, M. J. | Sharifah Hamid | Samuel-Verghese, S. | Yeang, Andhy
An indirect enzyme linked immunosorbent assay (ELISA) was used to show that proteins eluted from different samples of natural rubber latex gloves contained different relative proportions of various antigens as defined by + monoclonal antibodies directed against B-serum and C-serum proteins. Thus some gloves with low total protein content were shown to have a high proportion of one of these antigens, but negligible amounts of other antigens, while some other gloves had high+ levels of more than one antigen tested. These differences become important if some of these antigens are more allergenic than others, and this report highlights the inadequacy of total protein quantitation in the absence of specific immunoassays as a + measure of the safety of a product.
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