The microstructure of the rambutan (Nephelium lappaceum L.) embryonic axis
2003
Chua, C.K. | U.R. Sinniah | A.G. Yunus | M.S. Sanusi | A.R. Razak
Cryopreservation has become a promising tool for long-term conservation of plant genetic resources, especially for plants that produce recalcitrant seeds. The success of cryopreservation is largely dependant on the water content of the tissue. Hence, material to be cryopreserved has to be sufficiently desiccated.The method commonly used is dehydration of zygotic embryonic axes prior to their introduction into liquid nitrogen. Zygotic embryos of recalcitrant species, which usually make up only a tiny fractionof the entire seed volume, are most often sufficiently small to be rapidly and evenly dehydrated and to be successfully cryopreserved. However, only very limited success has been achieved to date withtropical fruit species. The present study attempts to understand the nature of the embryonic axis in relation to the cotyledonary tissues in Nephelium lappaceum, an important tropical fruit species.Longitudinal and transverse sections of the rambutan embryonic axis were viewed under scanning electron and light microscope. Construction of the tissues namely the epicotyl, radicle and procambium, in relation to the cotyledon are discussed. A three-dimentional construct of the embryonic axis will be presented.
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