A fruit specific and ripening related endopolygalacturonase gene from papaya (Carica papaya L.)
2003
Roohaida O. | Tan W.F. | Zainon M. A. | Hamid L.
Fruit ripening is caused by a series of cordinated physiological and biochemical processes resulting in changes in its texture. Fruit softening is associated with extensive solubilization and degradation of cell-wall polysaccharides, which result from the action of a number of cell-wall hydrolytic enzymes including polygalacturonase. Endopolygalacturonase has been extensively studied in tomato fruit where it accumulates during ripening and is responsible for the degradation of polyuronides in the fruit cell wall. A fruit specific and ripening related endopolygalacturonse clone 2.5kb in length has been isolated from papaya cDNA library using differential screening method. The papaya endopolygalacturonse transcript showed increasing expression during fruit ripening but was undetectable in other tissues hence indicating its specificity in repening fruit tissues.
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