Development and characterization of iron- and zinc-biofortified rice events in NSIC Rc 238 background
2019
Bueno, G.M.S. | Soriano, J.C.V. | Tsakirpaloglou, N. | Trijatmiko, K.R. | Slamet-Loedin, I.
Iron (Fe) and zinc (Zn) deficiencies are highly prevalent micronutrient deficiencies affecting billions of people worldwide. Fe deficiency is the major cause of anemia while Zn deficiency is the major cause of stunting in children. Rice biofortification is one sustainable approach to help alleviate Fe and Zn deficiencies. In this study, 443 transgenic events in NSIC Rc238 background were generated and screened for single-copy insertion, high Fe and Zn content, and good grain yield. The 6 best-performing single-copy high Fe- and Zn- rice events from 2 constructs containing a gene encoding Fe storage-protein from common bean or apple and a gene encoding enzyme involved in the biosynthesis of Fe and Zn chelator from rice were evaluated under confined test (CT) conditions to select the best event for product development. Based on high Fe and Zn grain levels as well as good agronomic performance shown during CT2017, 3 best-performing events (Rc238-IRS1030-008, Rc238-IRS1030-031 and Rc238-IRS1030-039) were selected and further evaluated in CT2018. These 3 events showed significantly higher Fe and Zn concentrations in polished grains (9-14 ppm Fe and 20-42 ppm Zn) compared to control (2-4 ppm Fe and 15-18 ppm Zn) and having no significant difference in yield. Further evaluation is currently underway in CT2019 to select the best event for the development of high iron and zinc rice.
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