Molecular identification and preliminary genetic diversity analysis of tobacco stemborer, Scrobipalpa aptatella (Walker) (Lepidoptera:Gelechiidae) from Northern Mindanao [Philippines] using cytochrome c oxidase I
2019
Ogot, A.V. | Latina, R.A. | Caoili, B.L.
Scrobipalpa aptatella (Walker) or tobacco stemborer (TSB) is a reported pest of tobacco worldwide. Its damage is characterized by stem tunnels created by larval feeding that consequently result to stem galling and wilting as the infestation progresses. Proper identification of TSB is crucial in establishing effective control measures to prevent serious infestation. DNA barcoding was used to establish the identity of TSB infesting tobacco plantations. Using cytochrome c oxidase I (COI) gene as the molecular marker, TSB samples collected from Claveria, Misamis Oriental and Bukidnon [Philippines] revealed 99.0% nucleotide similarity to S. aptatella (Walker) COI sequence (GenBank Acc. No. KF388766). Multiple alignments of the COI sequences showed single nucleotide polymorphism within and between the collected populations. Haplotype and nucleotide diversity analyses resulted to values of 0.2584 and 0.00074, respectively that revealed five haplotypes. These haplotypes showed distinct separation in the phylogenetic tree constructed. Evidence of TSB population expansion was also observed based on Tajima's D and Fu's tests of neutrality. The occurrence of S. aptatella in these production areas calls for the development of control measures against TSB infestation.
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