Identification and validation of mango varieties in the Philippines using Simple Sequence Repeats (SSRs)
2019
Rama, R.A.B. | Vera, S.V.I. | Espino, M.R.M. | Espino, R.R.C.
Mango (Mangifera indica L.) is the third most important fruit crop in the Philippines. Among the different varieties present in the country, 'Carabao' is the most important. It accounts for the majority of hectarage and production in the country and backbone of our mango export. A major issue challenging the industry is access to quality planting materials including its varietal true identify being sold by various nurseries in the country. In addition, the Bureau of Plant Industry through the National Seed Quality Control Services (NSQCS) is mandated to undertake a plant material certification program. The use of molecular markers for varietal identification can facilitate plant varietal certification by authenticating the variety being sold by these nurseries to interested buyers. In these study, twenty (20) SSR markers were screened across major varieties in the Philippines ('Carabao', 'Pico', 'Paho', and 'Kachamita'). Five (5) markers, namely Min256, Min346, MishRS39, MillHR30, and LMMA09, can successfully fingerprint these varieties. Furthermore, these markers were able to discriminate 'Corcino' and 'Prima' from other NSIC-registered 'carabao' mango strains. Planting materials from accredited and non-accredited nurseries were compared to reference variety obtained from foundation trees of BPI National Mango Research Development and Production Support Center. Results show that in some cases the identify of planting materials as per declaration of nurserymen do not conform with the DNA fingerprints of the reference variety. This was on some seedlings of 'Carabao' strains claimed to be 'Corcino', 'Prima', 'Lamao No. 1' and 'GES77', all are NSIC-registered varieties.
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