Optimisation Оf The Microdilution Method For Detection Of Minimum Inhibitory Concentration Values In Selected Bacteria
2016
Kolarević, Stoimir | Milovanović, Dragana | Avdović, Merisa | Oalđe, Mariana | Kostić, Jovana | Sunjog, Karolina | Nikolić, Biljana | Knežević-Vukčević, Jelena | Vuković-Gačić, Branka
In this study we investigated the influence of preparation of the bacterial inoculum for a microdilution susceptibility test, e.g., the effect of its optical density, on assessment of the minimum inhibitory concentrations (MIC). The approach employed in the majority of microdilution susceptibility studies is use of the same optical density for preparation of inoculums for different bacterial strains. In the present work, this approach was questioned by determining the ratio between the optical density and the number of bacteria in cultures. We also investigated whether the number of bacteria in inoculums can affect assessment of the MIC value for two antibiotics of broad spectra, rifampicin and streptomycin.
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