PHYSIOLOGICAL PARAMETERS AND ANTIOXIDANT ACTIVITY OF CELLS OF PHOTOMIXOTROPHIC CALLUS CULTURE TRIGONELLA FOENUM-GRAECUM L.UNDER THE INFLUENCE OF SELENIUM
2021
Logvina A.O. (Belarusian State University)
The current study presents the results of determining the growth parameters, the content and ratio of photosynthetic pigments,the level of accumulation of phenolic compounds and antiradical properties of cells of a photomixotrophic callus culture offenugreek (Trigonella foenum-graecum L.) when an inorganic source of selenium (Se) in the form of sodium selenite inconcentrations of 10-8-10-3 M is introduced into the nutrient medium.It was found that the concentration of selenite equal to10-3 M is toxic for the callus tissue. This is indicated by the complete inhibition of the growth processes of the culture as well aschanges in the content and ratio of photosynthetic pigments, which are evidence of significant suppression of the photosyntheticapparatus. At lower concentrations of the exogenous substance, there is a significant response from the physiological parametersof the callus culture or there is no response (10-8-10-5 M), or negative consequences relate only to growth processes (10-4 M)without affecting the functioning of the pigment system of the photosynthetic apparatus. A slight decrease in the level ofphotosynthetic pigments occurs when introducing 10-5 and 10-3 M of the tested substance. The positive correlation between thecontent of phenolic compounds and the antiradical activity of water-alcohol extracts of callus tissue with varying concentrationsof sodium selenite in the nutrient medium indicates a significant role of these metabolites in the manifestation of the antioxidantproperties of the object
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