???????????????????????????????????????????????????????? 18 ?????????????????????????????????? SRAP | Genetic diversity of the 18 commercial waxy corn cultivars based on SRAP markers
2019
Buppa Kongsamai(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Premjit Leangumnuay(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Agricultural Biotechnology Program) | Panalee Pooworakulchai(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Horticulture) | Anyamanee Auvuchanon(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Horticulture)
This study was to evaluate genetic diversity of 18 varieties of waxy corn, including 2 hybrid varieties, 13 open-pollinated varieties and 3 exotic varieties compared to 2 varieties of sweet corn by using SRAP markers (Sequence-Related Amplified Polymorphism). It was found that 12 primers provided 61 polymorphics of DNA band. Analysis of genetic relationship and cluster by using Unweighted pair group method with arithmetic averages (UPGMA) showed that the waxy corn and the sweet corn could be grouped according to Dice's similarity coefficient (0.35-0.76) into 4 groups. Group 1 included 2 varieties of hybrid waxy corn 'Violet white-926' and an exotic variety 'Chinawax-23'. Group 2 included commercially hybrid sweet corn (Supersweet-3A and Supergold) and F1 hybrid variety of waxy corn (Bigwhite#854), open-pollinated varieties (White Hawk, Hueplee-20, Snowwhite-seedlines, Pum-pui-Singto and Khaw-niuw-wan-14) and two exotic varieties (Chinawax-2 and Chinawax-2000). The open-pollinated varieties of waxy corn, which were consisted of Kai-mook-Pum-pui, Nakhon Pathom, Eight-row and Snowwhite) were grouped into the third group. Group 4 had only 2 varieties of open- pollinated waxy corn with a small ear type; Khao-kawniuw and Num-Nan. But there were 2 cultivars with small ear type in out group that were Numyom and Numwang. The result of the study indicates that there is genetic variation among the waxy corn varieties evaluated, which is able to select the parental lines for developing a breeding population
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