Isolation and Characterization of a Putative Ammonia monooxygenase (amo) gene of Morganella morganii (Fulton, 1943) from Seafood Processing Waste Water
2018
Thakur, Abhishek | Lekshmi, Manjusha | Kumar, Sanath | Nayak, Binaya Bhusan
In the aquatic environment, microbial oxidation ofammonical nitrogen is an important biologicalprocess. The bacterial enzyme ammoniamonooxygenase is the key enzyme involved in theoxidation of ammonia. In this study, the ammoniamonooxygenase (amo) gene from Morganella morganii(Fulton, 1943) was isolated and characterized byfull-length gene amplification, cloning, sequencingand in-silico protein structure and function predictions.The nucleotide sequence of ammoniamonooxygenase gene was derived which showed99% similarity with the homologous gene of M.morganii sub sp. morganii. In-silico structural andfunctional analyses of the ammonia monooxygenasegene revealed a class of transporter protein with aputative role in ammonia transportation. Furtherstudy is necessary to understand the role of thisprotein in ammonia oxidation by M. morganii.
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