Application of new high-performance liquid chromatography and solid-phase extraction materials to the analysis of pesticides in human urine.
2017
POZZEBON, J. M. | QUEIROZ, S. C. do N. de | MELO, L. F. C. | KAPOR, M. A. | JARDIM, I. C. S. F.
A method for the simultaneous determination of diuron and linuron pesticides in human urine was developed, using both solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) phases made in our own laboratory. These materials were prepared by sorption of polysiloxanes onto a silica surface, followed by immobilization. The HPLC columns were prepared from poly(methyloctylsiloxane), PMOS, immobilized onto silica with microwave radiation while the SPE cartridges where made with poly(methyloctadecylsiloxane), immobilized thermally. Method validation was performed for diuron and linuron for three fortification levels. The recoveries obtained were 85?103%, the inter- and intra-assay precisions were less than 1.6 and 1.8%, respectively. The limits of quantitation and detection for diuron were 2.4 and 8.0 ?g/l and for linuron were 5.0 and 12 ?g/l, respectively.
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