Persistence of Xanthomonas campestris pv. campestris in Field Soil in Central Europe
2021
Filip Gazdik | Samuel Magnus | Steven J. Roberts | Rafal Baranski | Jana Cechova | Robert Pokluda | Ales Eichmeier | Dariusz Grzebelus | Miroslav Baranek
Xanthomonas campestris pv. campestris (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses of brassica crop production usually result from seed-borne infection, but carry-over of inoculum in field soil may also be possible. The aim of this study was to monitor persistence of Xcc in field soil in central Europe using a conventional PCR assay with hrpF primers and a two-step nested real-time PCR assay using Zur primers. The work has demonstrated that nested real-time PCR can be used to improve the analytical sensitivity for detection of Xcc in soil compared to conventional PCR, and that Xcc may persist in soil for up to two years following an infected brassica crop in central European climatic conditions.
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