Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy
2022
Manzulli Viviana | Rondinone Valeria | Serrecchia Luigina | Petrella Antonio | Scaltrito Domenico | Marino Leonardo | Pace Lorenzo | Luigia Prencipe Maria | Cipolletta Dora | Nitti Mauro | Fasanella Antonio | Poppa Elena | Galante Domenico
Brucellosis is a widespread zoonosis of great economic importance for livestock farming in many areas of the world. It is a highly infectious disease which is diagnosed using conventional serological and microbiological methods. The aim of this study was to assess the efficiency of a specific real-time PCR in combination with broth cultivation in detecting Brucella spp. in organs of infected cattle, in order to compare the sensitivity of the two approaches and the time needed in them until a correct diagnosis is made.
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