Biochemical reactions of barley leaves at intervals after inoculation with Erysiphe graminis f. sp. hordei
1986
Song, D.U. | Cho, B.H. | Kim, K.C. (Chonnam National Univ., Kwangju (Korea R.))
Papilla and cytoplasmic aggregates clearly formed on the epidermal cells of barley leaves in response to the primary germ-tubes of Erysiphe graminis f. sp. hordei, but their sizes were much smaller than those in response to the appressoria. Some cells of barley leaves exposed to powdery mildew for 36 - 48h were more deeply stained as compared to the other cells by acid fuchsin. However, the content of malondialdehyde in powdery mildewed leaves, one of the product of lipid peroxidation, did not increase by 96h after inoculation. Positive reactions for callose, protein and phenolics were recognized in the papilla and cytoplasmic aggregates at 6h after inoculation, but cutin, suberin, cellulose and lignin were not noticeable until 72h after inoculation. The total phenol content in methanol extracts increased with increasing tim0932e after inoculation. All histochemical reactions were not race-specific in barley powdery mildew combinations te
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