Systems for phage typing of Staphylococcus aureus of animal origin
1986
Ismaiel, A.K.M
541 isolates of Staphylococcus aureus from local animals (sheep, cows, mules, horses, dogs, goats, poultry) were thoroughly studied, specimens were mainly milk from clinical and subclinical cases, nasal swabs, wound swabs and abscesses. The biochemical and enzymatic characeristics were described and showed some interesting features especially concerning the coagulase test and colony pigmentation of the isolates. Sensitivity to 16 different antibiotics were ckecked and more than 80% of isolates were sensitive to eight of the different antibiotic used. In phage typing a total of 30 phages were used including the 23 phages of the human set and 7 phages of the bovine set suggested by Weybridge Institute. The percentage of the typability was different in these animals and showed the following consequence; cows 57.6%, sheep 32.5%, dogs 11.4%, horses and mules 6.4%, and stock 19.2%. Some isolates showed recognized typability with phages that were not suggested by other workers. Besides Routine Test Dilution (RTD-100) we also used the RTD-1000 especially for sheep and cows isolates; this increased typability 29.4% in sheep and 27.5% in cows
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