Enzyme-linked immunosorbent assay (ELISA) as a method for detection of Phytophthora fragariae Hickman in strawberry roots
1988
Werres, S. (Hannover Univ. (Germany, F.R.). Inst. fuer Pflanzenkrankheiten und Pflanzenschutz)
ELISA was utilized to detect Phytophthora fragariae Hickman in roots of strawberries in experiments under controlled and field conditions. First the specifity of the antiserum was examined. The antiserum was not specific in its reactions with different P. fragariae isolates. Other Phytophthora species reacted with the antiserum but much less than P. fragariae. The tested Pythium species gave no ELISA values. Under controlled conditions detection of the pathogen in strawberry roots with ELISA was positive even if inoculum density of the substrate was low. Under field conditions P. fragariae could be detected in inoculated strawberry plants in spring and summer
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