Genetic variation of alfa-amylase levels among rye (Secale cereale L.) kernels, tested by gel diffusion technique
1991
Masojc, P. | Larsson-Raznikiewicz, M. (Sveriges Lantbruksuniv., Uppsala (Sweden). Inst. foer Kemi)
The investigation concerned individual kernels of two inbred lines of rye (Ot1-3 and 541), their F2 progeny and rye cv. Danko. Analysis of ungerminated grain harvested in four consecutive years showed that Ot1-3 represents a low and 541 a high alfa-amylase genotype. The distribution of the alfa-amylase activities among the F2 kernels suggests that two duplicate, dominant genes might be involved in the control of low alfa-amylase level in rye grain. The gel diffusion technique used is simple, quick and inexpensive, suitable for large-scale early screening of genotypes for alfa-amylase activities. A high correlation (r=0.94) between the alfa-amylase content in distal halves and in whole kernels of cv. Danko was found, which suggests that only distal halves of kernels could be used for the alfa-amylase assay.
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