Peanut chlorotic ringspot virus (PCRV), a newly discovered virus infecting peanut (Arachis hypogaea L.)
1994
Wagih, E.E. (Alexandria Univ. (Egypt). College of Agriculture. Dept. of Plant Pathology) | Melouk, H.A. | Sherwood, J.L.
A virus causing wide chlorotic ringspot (PCRV) associated with chlorotic line pattern and mottling on an Erictoides hybrid growing in USDA-OSU greenhouses, Stillwater, Oklahoma, was discovered. The virus was isolated and characterized and found to differ in symptomatology, host range and serological properties from all the previously described viruses infecting peanut, particularly those reported in the United States to be the most important ones, peanut mottle virus, peanut stripe virus, and tomato spotted wilt virus. The virus was transmitted by both mechanical inoculation and grafting to fourteen peanut cultivars causing identical symptoms to those originally observed on the Erictoides hybrid. In addition to peanut, the virus systemically infected Pisum sativum L. 'Little marvel' causing mainly mosaic and Lupinus albus L 'Tiftwhite' producing severe malformation and remarkable reduction in leaflet area. The virus did not infect many other plant species of which cowpea 'California blackeye' (Vigna unguiculata L.) and at least five cultivars of soybean (Glycine max L.) are known to be susceptible hosts to peanut mottle virus. Phaseolus vulgaris L. 'Topcrop' and Chenopodium amaranticolor Coste et Reyn were found to be two useful local lesion assay and diagnostic hosts for PCRV. The virus elicited necrotic local lesions on the first and chlorotic ringspots on the second. PCRV had a dilution end point between 10(-5) and 10(-6), thermal inactivation point between 55 degrees C and 60 degrees C, and longevity in vitro up to 6 days but not 7 days. Virus particles viewed by electron microscopy and the negative stain uranyl acetate were flexuous filamentous particles ranging in length from 750-850 nm
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