Measurement of the glucosinolate content in rapeseed using the TRUBLUGLU meter
1993
Tholen, J.T. (Wollongong Univ., NSW (Australia). Dept. of Chemistry) | Buzza, G. | McGregor, D.I. | Truscott, R.J.W.
A simple and inexpensive method for the estimation of total glucosinolate (GSL) content in rapeseed is described. It employs a newly developed portable reflectance instrument, the TRUBLUGLU meter. The assay is based on the selective hydrolysis of GSLs by endogenous myrosinase at pH9, followed by measurement of the released glucose using Clinistix strips. The meter, which is calibrated prior to use with a standard glucose solution, can display a digital readout in units of either mM glucose or mymol GSL/g seed.
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