RFLP-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.)
1993
Xie, D.X. (Cambridge Lab., Norwich (United Kingdom)) | Devos, K.M. | Moore, G. | Gale, M.D.
Thirty-eight anonymous cDNA and gDNA clone sequences from the libraries described in Devos et al. (1992) were shown to be located on the homoeologous group 5 chromosomes of wheat by nullisomic-tetrasomic analysis, and 26 of these were mapped. Two further anonymous cDNA markers, Xksu8 and Xksu26 (KamMorgan et al. 1989) and the cDNA clones containing the coding regions for ADP-glucose-pyrophorylase (Olive et al. 1989), alpha-amylase-3 (Baulcombe et al. 1987), beta-amylase-1 (Kreis et al. 1987), acyl carrier proteins 1 and 3 (Hansen 1987) and 2 (Hansen and Kauppinen 1991), a ubiquitin activating enzyme (Hatfeld et al.1990), a catalase (Bethards et al. 1987) and a bZIP protein (Guiltinan et al, 1990) also detected sequences on the wheat group 5 chromosomes. The genetic map position of the 18S.26S rDNA locus on the short arm of chromosome 5DS was determined using the clone pTa71 (Gerlach and Bedbrook 1979). The genetic location of a39 (PSR1201), a clone isolated using the ph1b mutant of Sears (1977) and mapping within the deletion on 5BL, was also determined. All DNA markers are presented with their chromosome arm location, copy number and relative hybridization strength in Table 1.
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