Induction of hairy and normal roots on Picea abies, Pinus sylvestris and Pinus contorta by Agrobacterium rhizogenes
1994
Magnussen, D. | Clapham, D. | Groenroos, R. | Arnold, S. von (Sveriges Lantbruksuniv., Uppsala (Sweden). Inst. foer Skogsgenetik)
Two- to three-week-old seedlings of Picea abies, Pinus sylvestris and Pinus contorta were inoculated with the supervirulent strain Agrobacterium rhizogenes R1600 2 cm above the base of the hypocotyl; other treatments included inoculation with Agrobacterium tumefaciens strain A281 (pTVK291) or mock inoculation. Hairy or highly branched roots formed on 2-3% of seedlings of all 3 species that were infected by R1600 but none of the other seedlings. Normal adventitious roots were also induced on 5-10% of Agrobacterium-treated seedlings but none appeared on the other seedlings. Opine assays and growth rates of hairy and normal roots indicated that only roots with the hairy morphology were transformed.
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